Sentence examples for brain was then placed from inspiring English sources

Each brain was then placed in a brain matrix, and coronal sections were cut into 2 mm slices.

The dura was carefully removed and the brains were then placed in ice-cold artificial CSF (aCSF) containing (in mM) 83 NaCl, 2.5 KCl, 3.3 MgSO4, 1 NaH26.2, 22.2 NaHCO3, 22 glucose, 72 sucrose, and 0.5 Cand2, and equilibrated with 95% O2/5% CO2.

Brains were then placed in 30% (w/v) sucrose for 5 days at room temperature in the dark, prior to sectioning in 6% (w/v) sucrose using a vibratome (200 µm, VT 1000S, Leica, Germany).

Brains were then placed in plastic blocks in insulin OCT (TissueTek) and frozen.

Brain tissue was then placed in autoclaved eppendorf tubes and kept on dry ice for immediate RNA extraction.

The hypothalamus was removed from the brain, which was then placed in a cooled matrix (ASI Instruments, Inc., Warren, MI).

A 5-8mm round coverglass (#1.5) was then placed with a layer of ACSF over the exposed brain.

The interim prosthesis was then placed intraorally.

The animal was then placed prone in an animal holder and the RF (radio frequency) coil was positioned and fixed over the brain of the animal.

The bottle was then placed upright.

The brain slices were then placed in medium containing 25% inactivated horse serum, 25% Hanks' BSS, 50% DMEM, and 25 mg/L penicillin-streptomycin (Invitrogen, Carlsbad, CA) in 6-well culture dishes and maintained for 30 minutes at room temperature.