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The brain was processed into celloidin-embedded serial sections stained with the Klüver Barrera and other conventional methods.
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For example, SNORD115, which is selectively expressed in the brain, is processed into shorter sequences lacking the stem sequence that is crucial for the assembly into a functional nRNP, but still containing the antisense box needed for targeting complementary RNA [ 129].
This means understanding not just how visual and other sensory information flowing into the brain is processed, but also how high-level knowledge, which experience has embedded deep in the brain, affects perception.
The perception of our environment is further facilitated by olfactory cilia, the membranes of which are filled with odorant receptor proteins that detect chemicals in our surroundings and send signals to the brain to be processed into our sense of smell (Jenkins et al. 2009).
Sagittal sections of the brains or heads were processed into paraffin blocks and sections cut at 6 μm.
The brains were processed routinely for paraffin embedding and sectioning into 5 μm thickness, and the sections were stained with hematoxylin-eosin (H&E) according to the methods described previously [ 21].
Dr. Michael Hansen, an expert on prion diseases at Consumers Union, described an experiment in which material to be processed into gelatin was "spiked" with infected brain.
The brains were processed as described above.
PND0 brains were processed as described for embryonic brains.
Brains were processed as previously described (55).
Brains were processed using standard methods.
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