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The whole brain was prepared for further analysis.
Microtubule protein from porcine brain was prepared by three temperature-dependent cycles of assembly and disassembly according to Shelanski [ 41].
Briefly, rats were sacrificed with CO2 and spinal cords were flushed out of the spinal column with sterile PBS, the brain was prepared and used in toto.
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Did you know that your brain was preparing for that movement a full half-second before it actually took place?
Slices of the rat brain were prepared at 10 to 40-μm thick using the Leica 1850 cryotome (Leica Instruments, Nussloch, Germany).
Serial frontal 4 µm sections including the entire brain were prepared.
Tissues extracts of liver and brain were prepared in the extraction buffer.
Twenty one days after intracranial implantation of tumors, mice were sacrificed and cryostat sections of brain were prepared.
Ten-percent (w/v) homogenates of mouse brain were prepared using a glass/Teflon apparatus (10 strokes at 1000 rpm) in ice-cold PBS.
Serial sagittal (lateral 1.70 1.00 mm) or coronal (A 4.70 mm–5.70 mm) cryostat sections (14 µm) of mouse brain were prepared at striatum level according to the atlas of Lehmann [56].
Serial coronal sections of formalin-fixed whole brain were prepared.
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