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Blood oxygenation level dependent (BOLD) fMRI in the brain was measured using T2*-weighted fast low angle shot (FLASH) imaging at 7T and was performed before, during and after CRD in rats with micropellet implants of CORT or cholesterol (CHOL) as a control.
The radioactivity present in blood and brain was measured using shielded well-type gamma scintillation counter.
The cross-sectional area of each slice of infarcted brain was measured using image analysis software (MetaMorph, PA, USA).
Photon emission from the whole brain was measured using Living Image 3.2 software (PerkinElmer) in a region of interest (ROI) that was kept constant across mice.
To verify that intranasally administered apelin-13 reached the brain, the apelin level in the brain was measured using Western blot assay.
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The intensity of the fluorescence in different area of the brain is measured using the signal intensity bar.
Regional kinetics of [11C]AF150(S) in rat brain were measured using a high-resolution research tomograph, both under baseline conditions and following pre-treatment with various compounds or co-administration of non-radioactive AF150(S).
The levels of PrPc in TgOvPrP4 and C57Bl/6 (Charles River, L'Arbresle, France) mouse serum or brain were measured using an ELISA (Enzyme Linked Immuno Sorbent Assay) method as previously described [65], [66], involving two different two-site immunometric assay based on two different combinations of monoclonal antibodies.
Total Hg concentrations in blood and total and inorganic Hg concentrations in brain were measured using a procedure adapted from Greenwood et al. (1977).
The area of Calyxes (dendritic structures of the Kenyon cells), Lobes (axon bundles of the Kenyon cells) and the size of the brains was measured using LSM Image software.
The AVT and IT concentrations in fish brains were measured using the procedure described by Kulczykowska (Kulczykowska, 1995).
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