Sentence examples for brain was isolated using from inspiring English sources
One microgram of total RNA from adult fly brain was isolated using TRIzol reagent (Invitrogen), converted to complementary DNA using SuperScriptII reverse transcriptase (Invitrogen), and then used for quantitative RT PCR with SYBR Green reagent (Applied Biosystems).
Total RNA from NL and BDL rat brain was isolated using TRIzol® Reagent in the PureLink™ Micro-to-Midi Total RNA Purification System Invitrogen™ Life Technologiess, Foster City, CA, USA) according to the manufacturer's instructions.
Mononuclear cells from the lung and brain were isolated using the same procedure that was previously described for the spleen.
Hippocampal mitochondria from rat brain were isolated using a Percoll (Amersham Biosciences, Piscataway, NJ) gradient centrifugation as described previously.
RNA from individual brains was isolated using the Qiagen RNeasy Mini Kit with the optional on-column DNase treatment according to the manufacturer's instruction.
Total RNA from each brain sample was isolated using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA), and RNA was quantified by OD 260.
Total RNA from the mouse brain homogenates was isolated using Sepasol (Nacalai tesque, Kyoto, Japan), according to the manufacturer's instructions.
Total mouse brain RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA).
Total RNA from brain cortices was isolated using the Tri Reagent Method (Sigma-Aldrich, St . Louis MO, USA), as described in the manufacturer's protocol.
Briefly, brains were dissected and total RNA was isolated using TRIZOL (Invitrogen, Carlsbad, CA), according to the manufacturer's specifications, and stored at −80°C.
Brain tissues were homogenized and total RNA was isolated using Trizol (Invitrogen).
