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The whole brain was irradiated using a field of 2 × 2 cm and a source-to-skin distance of approximately 99.5 cm to yield an absorbed dose of 8 Gy.
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Animals were anesthetized using an intraperitoneal injection of ketamine (75 mg/kg) and xylazine (6 mg/kg), immobilized in a customized jig and whole-brain was irradiated using 160 kV X-rays (Model CP160; Faxitron X-ray, Wheeling, IL, USA) as previously described.
A two-terminal photodetector was also fabricated and according to the absorption spectrum, the photodetector was irradiated using monochromatic light at 430 nm (0.4 mW cm−2).
Rat skin was irradiated using a NIR device.
Cells were irradiated using a Cs-137 irradiator (GSM GSR D1; dose rate 1.938 Gy min−1).
Control mice were irradiated using the same protocol but did not receive any human cells.
Cells were irradiated using a Caesium gamma source.
The second group comprised VAN specimens irradiated using US (VAN + US group) and, in the study group, VAN specimens were irradiated using MB-mediated US VANN + US + MB group).
Human keratinocyte cells and CHO-K1 mutant cell lines were irradiated using cobalt 60.
Wound beds were irradiated using a scanning technique with no direct contact.
The cells were irradiated using a caesium-137 source at 0.5/1 Gy, or mock treated.
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