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One-half of the brain was immediately cut on coronal sections, 1 cm thick, frozen in dry ice and stored at −80°C until use.
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The phone conversation was immediately cut off.
(Like THAT was gonna happen....) Anyway, I was immediately cut.
The brain was immediately removed, cut in standardized sections and fixed in formaldehyde.
When the riots began, communications were immediately cut off.
The samples were immediately cut into three parts.
The brain was immediately dissected, rapidly frozen in chilled isopentane, and cut to 20-μm cryosections at the levels of the FC and CB.
Brains were rapidly removed, cut along the midsagittal plane, and half the brain was immediately frozen and the other half drop-fixed in 4% paraformaldehyde for 48 h.
For in vivo measurements, unfixed brains were immediately removed, 100 μm sections were cut on a Vibratome in PBS at 37°C, collected in PBS at 37°C, first incubated in DMEM for 5 min at 37°C with 5% CO2, next in DMEM containing 3.33 μg/ml JC-1 for 30 min, and lastly in DMEM without JC-1 for 20 min, rinsed twice in PBS and mounted on glass slides with a coverslip in PBS.
The brains were immediately removed and snap frozen for post-mortem evaluation of brain inflammation.
Whole brains were immediately removed for processing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com