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Based on prior studies, we anticipated that the anterior corpus callosum would send projections to the anterior cerebral cortex whereas progressively posterior segments would send projections to more posterior cortex.A postmortem canine brain was imaged using a 7-T MRI system producing 100-μm-isotropic-resolution diffusion-tensor imanalyzedalyzed by tractography.
A 1 mm area of each of the PNGase F negative and positive parts of the brain was imaged using MALDI-MS separately.
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After sacrificing the mice, the whole brain was dissected and ultrathin sections (20 µm) of tumor bearing brain were imaged using the same fluorescence imaging system as used for in vivo.
Their brains were imaged using high-resolution diffusion tensor imaging (DTI) on a 7T small-animal magnetic resonance imaging system.
Stained whole brains were imaged using confocal microscopy (LSM 510).
Adult brains were imaged using a Zeiss 510 Confocal Laser Scanning Microscope (Carl Zeiss Microscopy, Jena, Thuringia, Germany).
Drosophila brains were imaged using confocal microscopy Nikon AIR confocal unit mounted on a TI2000 inverted microscope (Nikon Corp).
Also the radioactivity accumulation and distribution in the brain and the whole body was imaged using a PET camera, the results of which will be reported in separate publications.
For comparison, an adjacent acute brain section of the cortex was imaged using a single, intermediate excitation wavelength at 970 nm, which we empirically found to be the optimal intermediate excitation wavelength.
Brain sections were imaged using brightfield microscopy, and optical density of striatal staining was determined using ImageJ software.
For quantification of apoE/CAA or apoE/plaque co-staining in 10-month-old 5XFAD/apoEm/4 mice, brain sections were imaged using a Zeiss LSM 5 PASCAL system coupled to a Zeiss Axiovert 200 M confocal microscope.
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