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Acetylcholine level in the whole brain was estimated using Hydroxylamine method as described by Stepankova et al. [ 33].
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Total brain volume was estimated using Freesurfer (60).
The average number of Congo red-positive plaques in the APP/PS1 brain regions was estimated using the optical fractionator method (StereoInvestigator; MBF Science, Williston, VT, USA), as outlined previously [ 14].
The minimum cluster size of seven 3.75-mm voxels for a p <.05 whole-brain corrected threshold was estimated using AFNI's AlphaSim (Cox, 1996).
The brain metastasis-free survival was estimated using the Kaplan-Meier method and patient groups were compared using the log rank test.
Data were normalized for individual variation in intracranial vault volume, which was estimated using Brain Extraction Tool (BET) from the FMRIB Software Library from Oxford University [40].
The lower limit of detection of PrPSc using the current protocol was estimated using pooled brain homogenates in order to avoid sample to sample variations.
Glucose transport was estimated using an in situ brain perfusion technique with radiolabeled glucose and brain vascular GLUT1 expression was detected with immunofluorescence.
Subsequently, total distribution volume (VT) for each ROI was estimated using 2TCM using the fitted delay and vB from the prior whole brain fit.
1/S' was estimated using BlastPhen.
RNA concentration was estimated using nanodrop spectrophotometer.
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