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Briefly, the brain was embedded in 4% oxidized agarose and cross-linked with a 0.2% sodium borohydrate solution (in 0.05 M sodium borate buffer, pH 9.0 9.5).
Each mouse brain was embedded in 3% agarose and 50 µm-thick, free-floating coronal sections were cut in the Leica VT1000S vibrating microtome.
The left hemisphere of the brain was embedded in tissue freezing medium (Triangle Biomedical Sciences, Durham, NC) and rapidly frozen in a 2-methylbutane/dry-ice bath.
Brains from WT and mutant animals were harvested and the unfixed brain was embedded in OCT, frozen on dry ice, sectioned at 15 µm on a cryostat (Leica), mounted on glass slides and stored at −80°C until used.
Frozen brain was embedded in cryomatrix compound (Thermo Fisher Scientific, Waltham, MA) prior to sectioning.
For sectioning, the brain was embedded in OCT compound (Sakura, Torrance, CA, USA) and sectioned in the sagittal plane.
Similar(50)
a, For evaluation of ischaemic brain tissues, 1-mm-thick serial coronal slices from the brain were embedded in paraffin.
For them, the notion of embodiment must, of course, capture how the brain is embedded in a body but also how this body is embedded in the broader environment.
So there are a lot of dimensions along which things could be similar and neurons may respond to similar things, but the brain is embedded in a three dimensional blob, so you won't be able to replicate all similarity spatially.
"The entire brain is embedded in a dense meshwork of serotonin-releasing fibers, but only a small part of the brain is directly involved in the feelings that we can consciously access be it sadness or joy," Janusonis told VICE.
The spinal cord and brain were embedded in paraffin and cut into serial 6-µm thick coronal slides.
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