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(C) Ex vivo rat brain was dissected into two hemispheres--the left hemisphere was cut into 40-μm thick sagittal brain sections and were scanned to reveal brain areas.

Each brain was dissected into 16 areas: spinal cord, medulla oblongata, pons, mesencephalon, thalamus, hippocampus, cerebellar vermis, cerebellar medulla, cerebellar hemispheres, occipital cortex, temporal cortex, parietal cortex, striatum, frontal cortex, piriform lobe and olfactory bulbs.

Each brain was dissected into three parts: left and right hemispheres and cerebellum.

The brain is dissected into 528 coronal tissue sections at 25 μm separation, which are Nissl-stained, registered to each other, and assembled into a reconstructed brain volume.

Where indicated brains were dissected into striatum, hippocampus and midbrain.

Brains were dissected into several compartments: Tumor, ipsilateral hemisphere, contralateral hemisphere and midbrain.

Control or heat-shocked rat brains were dissected into cerebral cortex, hippocampus, midbrain, and spinal cord.

Brains were dissected into 1 mm thick coronal slices and immersion fixed in 4% paraformaldehyde in 0.1 M PBS overnight at 4°C.

Briefly, brains were dissected into 2.5% paraformaldehyde, 1% glutaraldehyde, 1% sodium metabisulfite in 0.1 M cacodylate buffer, pH 7.2, microwaved with a Pella research-grade oven (# 3450, with variable power controller #3430 and cold spot) with chilling plate at 18°C and power setting #2 for 2 min on/2 min off/2 min on/2 min off, and then fixed overnight on a shaker at 4°C.

Flies were anesthetized on ice, and brains were dissected into cool AHL.

Brains were dissected into cortical, hippocampal or striatal blocks and embedded in Epon resin.