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The expression of Rapamycin regulated genes in the brain was assessed using Agilent Custom arrays followed by Significance Analysis of Microarrays (SAM) analysis of the 1165 identified rapamycin-regulated genes only.
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Differences in the amount of Aβ in control and AD human brain were assessed using two-tailed student t-tests.
Expression of miR-21 in mouse cell cultures and mouse brain were assessed using Northern blot analysis and in situ hybridization.
The expression of genes from individual brains was assessed using the double-spotted A. mellifera brain 9 K version 3.0 cDNA microarray [ 17].
The expression of genes from individual brains was assessed using the double-spotted Apis mellifera brain 9K version 3.0 cDNA microarray using the protocols described by Whitfield et al. [ 11], Grozinger et al. [ 18] and Cash et al. [ 12].
They were then challenged with risperidone and brain activation was assessed using c-Fos immunohistochemistry. Risperidone significantly increased c-Fos expression in various brain regions (Figure 1 and Supplementary Table 1).
The distribution of brain perfusion was assessed using N-isopropyl p-[I]-iodoamphetamine (IMP) and SPECT.
Altered topology in individual brain regions was assessed using degree, strength, betweenness centrality and clustering coefficients.
General brain morphology was assessed using hematoxylin and eosin staining with a kit-based approach (Sigma-Aldrich Corporation, St Louis, MO, USA).
The current study investigated the neural processing of compounds with systematically varied psycholinguistic features, which were presented auditorily to participants whose brain activity was assessed using EEG.
Transport of PrioV3 and ICSM35 antibodies across the blood-brain barrier was assessed using GPNT rat [30] and HCMEC/D3 human [31] endothelial cells.
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