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The wolf brain was assayed for rabies antigen by fluorescent antibody (Public Health Laboratory, Springfield, IL).
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To estimate the minimal virus load of the mice, cohorts of the mice fed to cats were euthanized at the same time and frozen at –70°C; later, their brains were assayed for virus by plaque assay.
When brain tissue was assayed for DNA methylation, the genes showing the most clear-cut dual methylation profiles in the MAUD assay were those showing monoallelic expression in all the clonal lines (Figure S2).
Where two copies of a gene had been characterized, in some cases, only one was highly expressed in the brain and this was assayed for a LOF phenotype.
Mouse snoRNA135 expression was assayed for normalization.
Medium supernatant was assayed for Gluc activity.
Six brain sections were assayed for each gene category.
Brain extracts were assayed for ATP, phosphocreatine and lactate enzymatically (Williamson and Corkey, 1979).
Blood and brain samples were assayed for RDX content by the U.S. Army Public Health Command Directorate of Laboratory Sciences using gas chromatography with electron capture detection (GC-ECD) (Bishop et al. 2003).
Brain, heart, liver and spleen were assayed for hsp70, hsp90α and hsp90β mRNA.
Aliquots of brain homogenates and cell lysates were assayed for protein concentration by a BCA Protein Assay Kit.
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