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The fluorescent visualisation of the whole rat brain was achieved using a simple low power handheld UV lamp [58].
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Further investigation of the C-terminal-specific POMs was achieved using brain homogenates of transgenic mice expressing N-terminally truncated PrP variants, designated ΔE with deletion of 33 121 amino acids and ΔF with a longer deletion (33 134) which includes the hydrophobic central domain of PrP [19].
Brain-specific disruption of PEX13 was achieved using Nestin-Cre transgenic mice (Nes-Cre/+) in which Cre recombinase is expressed in cells of neuronal lineage under the control of the nestin promoter and intron 2 enhancer (Tronche et al., 1999; Zimmerman et al., 1994).
In this method, accuracy of 95% was achieved using skull stripping which eliminated all non-brain tissues for the detection purpose [1].
Densitometry quantification was achieved using ImageJ software.
Hierachial clustering was achieved using DiffBind31.
This was achieved using our Mantle toolkit.
This was achieved using the following procedure.
Normalization was achieved using SNORD47.
In the brain, this has been achieved using gradient index (GRIN) lenses for deep brain imaging, up to about 1.5 mm below the surface of the cortex.
This is achieved using the Brain suite[30] automated software package for skull removing.
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