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To study cerebral hemodynamics, all patients underwent duplex scanning of the neck and brain vessels using the scanner ACUSON 128XP/10c (USA) according to the standard method.
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To verify this finding by an alternative method, we performed biochemical enrichment of Notch3-ECD from isolated brain vessels using sequential protein extraction based on a protocol established previously [ 30].
Catheter surgery is one of the surgical methods for brain, vessel, and heart surgery in which doctors insert medical tools through the vessels using catheter tubes.
In this study, video images of brain vessels were used to study leukocyte-endothelial cell adhesion in single cerebral blood vessels in living mice (Supplementary information, Figure S1).
LAT1 protein levels in brain vessels were studied using LAT1 immunofluorescence.
Thus the neural tissue likely creates angiogenic gradients that blood vessels use to properly grow within the developing brain.
The mean and standard deviation was measured four times, as follows: (1) all voxels inside the brain mask determined by using the Otsu's thresholding technique, without removal of the CSF or vessels; (2) the brain mask after CSF voxel removal; (3) the brain mask after vessel voxel removal; and (4) the brain mask after removal of both the CSF and vessel voxels.
MRA showed segmental vasoconstriction of brain vessels allowing the diagnosis reversible cerebral vasoconstriction syndrome (RCVS).
Brain vessels provided the most prominent example.
To determine if the injected scFvs antibodies were able to localize on brain vessels, streptavidin-FITC was used and vessels were stained with anti-vWF revealed with anti-rabbit-TRITC.
In his current role at Duke, Yao and his team are using the technique for functional brain studies to examine neurons, blood vessels and other structures in the brain.
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