However, after correction of Kp,brain for brain tissue uptake (using Vu,brain derived from the brain slice method) and plasma protein binding (using fu,plasma) the BBB net flux was estimated as a 0.14 meaning that only 14% of unbound drug in plasma is passing the BBB.
Tracer uptake in the various brain structures were quantified using the standard brain atlas of the MI-Neurology tool of the syngo.via software (Siemens Healthcare GmbH, Erlangen, Germany), allowing the assessment of the relative mean tracer uptake of the brain regions as referenced in Table 1.
Global uptake differences between brain scans were adjusted using the "proportional scaling" statistical parametric mapping (SPM) option.
To answer this question, we intravenously injected 11C-LY2428703 into rhesus monkeys and healthy humans and quantified brain uptake relative to the delivery of radioligand to the brain via arterial plasma (i.e., using compartmental modeling).
The brain uptake of the IgG fusion proteins, 2 3% of injected dose/brain, is comparable to the brain uptake of small molecules.
A11 Influence of a genetic polymorphism on brain uptake of the dual ABCB1/ABCG2 substrate [11C]tariquidar.
At baseline conditions, the guinea pigs showed distinguishable brain uptake compared to the surrounding tissue, whereas low brain uptake was observed in the rats and mice (Figure 1).
In vivo11 C]N-methyl-SSR504734 showed high brain uptake; however, PET evaluation using nonhuman primates were not so far reported in the literature [23].
Finally, we describe how we initially assess preclinical radiotracer imaging, using brain uptake, specific binding, and preliminary kinetic analysis to identify promising radiotracers that may be useful for human brain imaging.
Note the similar cardiac uptake, but diminished mouse brain uptake potentially related to the use general anesthesia.
