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The DB29 and DB33 human glioma cells were established in the laboratory by culturing brain tumour samples obtained from the tissue repository at Methodist Research Institute (Indianapolis, IN, USA) with an IRB approved protocol.
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Brain tumour samples were obtained from 35 patients (15 females and 20 males) with either MBs or supratentorial PNETs who were operated on at the University Hospitals of Tampere and Turku, Finland, from1989-2005.
In order to obtain these brain tumour samples, however, you would need to go for surgery again.
DB29 primary human glioma cells were established in our laboratory by culturing surgically removed brain tumour tissue samples obtained from the tissue repository at Methodist Research Institute with an IRB approved protocol.
Brain tumour samples were fixed in 10% neutral-buffered formalin (Sigma) and embedded in paraffin.
The pooled brain homogenate was prepared from obex brain samples obtained from two CWD-positive Wisconsin hunter-harvested deer.
Primary tumour samples were obtained from brain tumour patients with informed consent and were intraoperatively frozen in liquid nitrogen (for RNA isolation) or fixed in 4% formaldehyde (for histology).
Tumour samples were obtained from primary tumours and skin, lymph node, soft tissue, lung, visceral organ and brain metastases.
Seven days later, surgery was performed and tumour samples were obtained for histology and autoradiography.
Human tumour samples were obtained with the corresponding written consent given by the patients.
Tumour samples were obtained from the Department of Pathology, National University Hospital of Iceland.
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