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Cells and mouse brain tissues were processed as previously described [1], [20].
Brain tissues were processed and immunostained as described.
Rodent brain tissues were processed for hematoxylin eosin (H&E) and Congo red staining.
At 3 months post-transplantation, the mice were killed and the brain tissues were processed for human-specific cell identification.
Adjacent coronal cryostat sections of rat and human brain tissues were processed at −20°C and were thaw-mounted on superfrost plus microscopic slides, dried and stored as described earlier [ 54, 64].
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Brain tissue was processed as previously described [47] (see Materials and Methods S1).
Human brain tissue was processed as described [13].
Mouse brain tissue was processed as described [34] and staining procedure for human tissue was applied.
Mice were then sacrificed and their brain tissue was processed for Nissl staining.
Local research ethical approval and full consent for brain tissue retention and research was obtained and the CNS tissues were processed by the Newcastle Brain Tissue Resource (NBTR).
Fixed brain tissue was trimmed into 5 standard transverse sections [ 39] and tissues were processed by routine histological methods and embedded into paraffin blocks.
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