Sentence examples for brain tissues were prepared from inspiring English sources

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In the present study brain tissues were prepared from patients with patients DNTC, calcified and non-calcified Alzheimer's disease (AD) patients.

Briefly, 20% homogenates of brain tissues were prepared and cultured in Vero cells for 14 days.

Harvested cell pellets and mouse brain tissues were prepared as previously described.

One week after ICV injection, animals were examined in the Morris water maze or sacrificed and brain tissues were prepared for TUNEL staining, immunofluorescence, and Western blot.

Briefly, 10% (w/v) brain homogenates from brain tissues were prepared in Dulbecco's sterile phosphate buffered saline (PBS) lacking Ca2+ and Mg2+.

SH-SY5Y cells and brain tissues were prepared for immunogold labeling TEM by minimal, cold fixation and embedding protocols, as previously described in Soura et al., (2012) [ 33] and Thorpe et al., (2001) [ 34], respectively.

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PrPres-enriched samples of brain tissue were prepared using a previously described procedure [23].

Briefly, single cell suspensions of brain tissue were prepared by treatment with DNase-I (28 IU/ml; Sigma- Aldrich) and collagenase (0.5 mg/ml; Sigma- Aldrich) enzymes for 1 hr at 37°C under frequent agitation and trituration.

Protein samples from brain tissue were prepared as a 10% brain homogenate, and tissue specific galectin-3 protein was detected using antibody specific for human recombinant galectin-3 (Abcam Inc, Cambridge, MA) and a commercially obtained chemiluminescence-linked western blot kit (Western Light Tropix, Bedford, MA).

Concerning the application to biological samples, sections (20 µm) of fresh mouse brain tissue were prepared.

To detect levels of PrPsc protein accumulation in the spleen and brain, 20% solutions of spleen and brain tissue were prepared in 0.01M TrisHCL pH 7.4, 0.005 M MgCl, treated with DNAse, and sonicated.

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