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Samples of both the spleen and brain tissues were pooled using equal amounts of total RNA.
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Homogenate was incubated at 37°C for 24 h then centrifuged at 16000 g for 30 min. Pellets derived from 1 g of brain tissue were pooled and resuspended in 3 mL of deionized, ultra-pure H2O and were dialyzed against three changes of deionized, ultra-pure H2O (MWCO 10 12 kDa) over 24 h to remove small molecular weight contaminants.
Brain tissue was pooled from cerebreum and cerebellum from two macaque females and spleen tissue was pooled from four macaque females and one macaque male.
Tissues were pooled according to sex.
The brain tumor tissues and contralateral normal brain tissues were collected, pooled at five per group, and subjected to apoptotic assay by Western blot for cleaved 89-kDa fragment of poly-ADP-ribose-polymerase (PARP) as a marker of apoptosis.
Tissue samples from gill, liver, spleen, kidney, heart, muscle, and brain were homogenized separately, then 20 μL of aqueous phase from each tissue was pooled to capture the maximum breadth of microbe diversity within each individual.
The brain tissues were removed immediately.
Tissue punches for corresponding brain regions were pooled, transferred to RNA later solution (Qiagen) and frozen for subsequent RNA extraction.
Hair and brain tissue were eliminated.
For neurosphere culture, brains from several pups were pooled together.
For the P30 animals, given that a single brain was selected per litter, the laser-excised hippocampal pyramidal tissues from the left hemisphere were pooled and used for assessing CGI methylation.
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