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Meanwhile, the release of nitric oxide (NO) and activities of NO synthase (NOS) in brain tissues were measured.
The levels of BDNF in brain tissues were measured using sandwich ELISA 7 days after MCAO.
To investigate the effects of NaB on lipid peroxidation and free radical activity induced by BCCAO, the contents of MDA and the activities of SOD in the brain tissues were measured.
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The lesion volume and the amount of histologically intact residual brain tissue were measured at the end of the experiment.
Total cerebral blood flow (tCBF) and total brain perfusion (tCBF per 100 ml brain tissue) were measured with 2D phase contrast imaging as described above.
Aβ42, P-tau, IL-6, and ACh levels in CSF, insulin levels in plasma, and Aβ42 levels in plasma and brain tissue were measured by ELISA.
Active GLP-1 concentrations in serum and brain tissue were measured by the Active form Assay Kit-IBL (Immuno-Biological Laboratories, Gunma, Japan).
Cytokine levels in the serum and brain tissue were measured by Bio-Plex Pro™ rat cytokine multiplex assay kit according to the manufacturer's instructions using the Luminex® 200™ system (Invitrogen, Carlsbad, CA).
30 minutes after the intravenous injection of EB, animals were carefully perfused to remove excess dye and the content of EB in the brain tissue was measured at 610 nm using a spectrophotometer.
Electrical conductivity of the brain tissue was measured during US exposure as a surrogate measure for tissue permeability.
The water contents of the brain tissues were detected by measuring the ratios of the body weights and brain weights.
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