Sentence examples for brain tissues were lysed from inspiring English sources
Frozen tumor and normal brain tissues were lysed in RIPA lysis buffer with protease inhibitors as described [34].
Rat brain tissues were lysed in homogenization buffer (20 mM Hepes, pH 7.6, 125 mM NaCl, 10% glycerol, 1 mM EDTA, 1 mM EGTA supplemented with 1% DTT).
(B ) WT mice were dosed once intraperitoneally with AMD3100 at 10 mg/kg body weight and 24 hr later, the brain tissues were lysed in NP-40 buffer with phosphatase inhibitors and immunoprecipitated ZBTB16 antibody.
MIN6 cells, mouse islets, mouse exocrine tissue and mouse brain tissue were lysed in cell lysis buffer (Cell Signaling Technology, Danvers, MA, USA).
In brief, brain tissue was lysed in Mg2+ lysis buffer (Upstate) and mixed with GST-PBD or with GST-RBD bound to glutathione-agarose and incubated for 1 h at 4°C.
5 10 mg of brain tissue was lysed using cell lysis solution (Qiagen) and Proteinase K (MP Biomedicals LLC) was added and incubated at 55°C over night.
Extracts of brain subicular tissues were lysed by sonication with PBS containing protease inhibitor cocktails (Sigma-Aldrich).
For detection of Prpc and anp32a, brain and spleen tissues were lysed in RIPA buffer (50 mM Tris HCl pH 8, 150 mM NaCl, 1% NP-40, and 0.5% sodium deoxycholate, 0.1% SDS) with protease inhibitors (Sigma P8340; Sigma, St . Louis MO) and centrifuged at 12000 rpm for 20 minutes.
Cells or tissues were lysed.
Mice tissues were lysed and sonicated in glycerol buffer [27].
