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The brain tissues were dehydrated with alcohol through and clearing them in xylene, which are finally embedded in paraffin wax (mp 58 60 °C).
To perform this experiment, fresh brain tissues were dehydrated by replacing the water with paraffin.
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Subsequently the brain tissue was dehydrated in ascending alcohol and embedded in paraffin.
Before embedding in paraffin wax, the tissues were dehydrated through graded alcohol.
The tissues were dehydrated using ethanol series and mounted with DePeX.
Then, tissues were dehydrated and embedded in paraffin.
After fixation, the tissues were dehydrated in graded ethyl alcohol concentrations and embedded in paraffin.
Tissues were dehydrated through a graded ethanol series, and embedded in Epon.
After washing in buffer, tissues were dehydrated in a graded series of ethanol solutions.
Floral tissues were dehydrated and included in Paraplast X-tra (Thermo, Waltham MA, USA).
Fixed tissues were dehydrated in an increasing series of ethanol and xylene and embedded in paraffin.
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