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Brain tissues were cryoprotected, embedded in OCT and frozen.
The brain tissues were cryoprotected by infiltration with 30% sucrose overnight.
Then brain tissues were cryoprotected after soaking in a series of sucrose solution (10%, 20%, and 30%) at 4°C until they sank.
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After fixation and dissection, the brain tissue was cryoprotected in 30% sucrose and sectioned on a freezing microtome in the transverse or sagittal planes into 40 µm-thick sections.
Brain tissue was cryoprotected and embedded in OCT, and sectioned at 14 µm parasagittally using a cryostat.
Tissues were cryoprotected in 15% to 30% sucrose solution and frozen on dry-ice.
Tissues were cryoprotected in 30% sucrose in 0.1 M phosphate buffer after immersion-fixation.
Then, the tissues were cryoprotected in 20% sucrose in PBS for 24 48 h at 4°C.
All tissues were cryoprotected with increasing sucrose concentrations and frozen in OCT.
Tissues were cryoprotected in 30% sucrose/phosphate buffered saline then mounted in optimum cutting temperature medium.
Tissues were cryoprotected in sucrose, embedded in TissueTek, and sectioned at 16 μm.
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CEO of Professional Science Editing for Scientists @ prosciediting.com