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Frozen brain tissues were weighted and homogenized in fivefold volume of 50% trichloroacetic acid solution.
Briefly, prior to elemental analysis, the brain tissues were digested with nitric acid (ultrapure grade) overnight.
At 60 hr after the return of spontaneous circulation, the pigs were sacrificed and brain tissues were harvested.
Brain tissues were homogenized with pre-cooled 1% polyvinylpolypyrrolidone solution (50 mM in pH 7.6 PBS) after isolation.
For immunohistochemistry tumor and brain tissues were fixed in formalin.
Mouse brain tissues were kindly provided by Prof. Orly Reiner.
U251 and 5310 glioma cells or brain tissues were harvested as previously described [58].
Human brain tissues were obtained from The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam.
The collected brain tissues were postfixed overnight in the same fixative solution.
Brain tissues were homogenized and total RNA was isolated using Trizol (Invitrogen).
Cells and mouse brain tissues were processed as previously described [1], [20].
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