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Entire brain tissues obtained from control and infected neonates at 7 d p.i. were dissociated into single cell suspension as using previously described methods [10].
To test whether ISG15 expression is also elevated in vivo, we first assessed the expression of ISG15 and its conjugates in various regions of brain tissues obtained from wild type and ATM knockout mice.
Ethics Statement: Written informed consent for the donation of adult tumor brain tissues was obtained from patients prior to tissue acquisition, under the auspices of the protocol for the acquisition of human brain tissues obtained from the Ethical Committee board of the University of Padova and Padova Academic Hospital.
To this end, we performed immunohistochemical analysis of γ-taxilin and αNAC using brain tissues obtained from patients with AD.
Five non-neoplastic brain tissues obtained from patients undergoing surgery for chronic epilepsy were included in the study as control samples.
This study measured electrical conductivities parallel and perpendicular to the pial surface in freshly excised brain tissues obtained from epilepsy surgery patients.
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Furthermore, Morg1 mRNA expression was significantly enhanced in the ischemic brain of WT, but not in ischemic brain tissue obtained from Morg1+/− animals.
On the other hand, ISG15 expression was modest in brain tissue obtained from normal individuals (lane 1 and 2).
Histopathological analysis of brain tissue obtained from individual patients who had died from unrelated causes revealed beta-amyloid plaque clearance in association with increased antibody titers, with some brain regions virtually free of beta-amyloid plaques [1].
In the current study, we show that ISG15 is elevated in A-T astroglial cells and brain tissue obtained from ATM knockout mice and patients (Figure 6 and 7).
Mitochondrial cytochrome oxidase protein levels are five-fold lower in gliomas compared to those of normal brain tissue obtained from epileptic patients (Griguer et al., 2013).
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