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Brain tissues from mice (n = 3/group) along the IBZ ipsilateral to the injury were extracted.
To gain an insight into the inflammatory milieu induced in the brains of APP/IFN-γ Tg mice after T cells had migrated to sites of Aβ plaques, we performed quantitative PCR analysis of brain tissues from mice that had been immunized with Aβ/CFA and co-injected with PTX.
In our laboratory, we have verified the specificity of this antibody through DAB immunostaining of lung and brain tissues from mice that express GFP under a GFAP promoter.
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Infected brain tissues from mouse scrapie, strain ME7, were obtained from FRCVD, Tubingen, Germany and mouse BSE, strain 301 V from VLA, Weybridge, UK.
The extracted brain tissue from mice injected with Apt-MNC was dehydrated in increasing alcohol concentrations, cleared in xylene, and embedded in paraffin.
Microscopic examination of brain tissue from mice infected with the Sterne strain showed thickening of the meninges, an influx of inflammatory cells and substantial hemorrhaging (Fig. 7D F).
In accordance with the extended survival, the viral titres in the brain tissue from mice treated with two doses of P-2 siRNA were reduced by 4logs as compared to the control group (p<0.00031) (Fig 8).
In TEM micrographs of brain tissue from mice that received an intratumoral injection of FE-Pro-labeled NSCs, we observed electron-dense FE-Pro complexes that were still internalized within the membrane-bound structures, demonstrating that the complexes were retained for at least 5 days post-labeling (4 days post-implantation) (Fig. 6A).
We thank: Dr. Dan McCormick and Jane A. Petersen for extending their technical expertise in synthesizing the Aβ40; and fluorescein labeled Aβ40 and 42; Geoffry L. Curran for his assistance in harvesting brain tissue from mice and 125I labeling of Aβ40; Jarred Nesbitt and Dr. Anthony Windebank for providing PC12 cells; and Jennifer Scott for her excellent secretarial assistance.
Second, LRP1 protein levels were dramatically higher in brain tissue from mice carrying orthotopic glioma tumours than normal mice.
For histological staining, brain tissue from mice at three different developmental stages was used: P7 (postnatal day 7) and P > 60.
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