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The scrapie inocula were prepared from brain tissues collected from terminally sick mice.
Gas-chromatography mass spectrometry (GC MS) analysis of brain tissues collected from untreated and treated PS1-KI and 3xTg-AD mice (n=3 for each study group) was performed as previously described.
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The team will use them to study the expression of approximately 25 genes in samples of postmortem brain tissue collected from very young children with autism.
To test this hypothesis, we combined microscopic MRE with brain tissue collected from adult male rats subjected to a controlled cortical impact injury.
This work specifically sought to identify disease-associated alternative splicing patterns in ion channel genes by comprehensively screening affected brain tissue collected from patients with mesial temporal lobe epilepsy and Alzheimer's disease.
Brain tissue collected from still births tested positive for the virus, as did spinal fluid from babies with microcephaly.
Brain tissue collected from the rat samples was homogenized in 0.075 M NaCl and 0.024 M ethylenediaminetetraacetic acid (EDTA) buffer, pH 7.5, at a ratio of 1 g of tissue to 1 ml of buffer, and then cooled to 4°C.
Using brain and gonad tissues collected from E10 to adult stages, Mank & Ellegren [ 11] computed the "amplitude" of gene expression on Z chromosome.
Therefore, an increased creatine to phosphocreatine ratio would be expected in brain tissue collected after a 2 or 30 min PMI, relative to a PMI of <0.5 min. Due to the complex and overlapping nature of absorbance bands in FTIR spectra collected from brain tissue, a direct measurement of the creatine to phosphocreatine ratio cannot be made.
In the present study we addressed these issues by examining the binding substrates of the highly fluorescent PiB derivative 6-CN-PiB in Alzheimer's disease post-mortem tissues collected from brain regions known to differentially accumulate neuritic and diffuse Aβ plaques and NFT, and to display differential PiB-PET retention levels in vivo (Klunk et al., 2004).
The tissues collected from cv.
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