Given these data, a study of the levels of expression of the 5-HT3Br1 subunit protein in brain tissues would be worthwhile, as the data showing high levels of 5-HT3Br1 subunit RNA in neurones may not provide an accurate picture of the relative proportions of different types of 5-HT3 receptor subunits being expressed.
In addition, the amounts of PrP Sc) in muscle were much lower than those in the brains of infected animals, and the team estimates that muscle tissue would be 5000 times less infective than brain tissue.
For the future goal of neural stem cell-based therapy in the brain, elasticity of less than 1 kPa, which is similar to that of the intact brain tissue, would have been ideal.
It is clear that there are tissue-specific, post-mating gene expression responses [compare [ 19, 22]; however, it is unlikely that changes in gene expression in head and brain tissues would have been detected by the previous whole animal studies given the sensitivity of the microarray assay [see [ 23, 24].
In this case, samples from the corresponding brain tissue(s) would be needed to be screened for epi-marks – requiring postmortem analysis of brains from suitably preserved cadavers of known sexual orientation.
This technique could not be applied to brains because brain tissue would have undergone severe anoxia during dissection.
The long-term implantation of electrodes into human brain tissue is already being done with deep brain stimulators that alleviate chronic pain and movement disorders.
The view of immunological ignorance of the CNS has, however, been in conflict with observations by Medawar and colleagues that an allogenic tissue graft into the brain, which would be tolerated in a naive host, was readily rejected in a recipient which was sensitized to the allo-antigens before the transplantation [ 2].
Given the direct connection of ocular tissues to the brain, the eye would be an attractive site for early diagnosis and monitoring of neurodegenerative diseases.
