Sentence examples for brain tissue were prepared from inspiring English sources

PrPres-enriched samples of brain tissue were prepared using a previously described procedure [23].

Briefly, single cell suspensions of brain tissue were prepared by treatment with DNase-I (28 IU/ml; Sigma- Aldrich) and collagenase (0.5 mg/ml; Sigma- Aldrich) enzymes for 1 hr at 37°C under frequent agitation and trituration.

Protein samples from brain tissue were prepared as a 10% brain homogenate, and tissue specific galectin-3 protein was detected using antibody specific for human recombinant galectin-3 (Abcam Inc, Cambridge, MA) and a commercially obtained chemiluminescence-linked western blot kit (Western Light Tropix, Bedford, MA).

Concerning the application to biological samples, sections (20 µm) of fresh mouse brain tissue were prepared.

To detect levels of PrPsc protein accumulation in the spleen and brain, 20% solutions of spleen and brain tissue were prepared in 0.01M TrisHCL pH 7.4, 0.005 M MgCl, treated with DNAse, and sonicated.

Protein extracts from primary cultures, HEK293T or brain tissue were prepared as described.

After a survival time of 7 8 days, the animals were perfused with a fixative and brain tissue was prepared for histological analysis.

Mouse brain tissue was prepared for histological analysis using standard techniques, as described [12].

ProSAAS mouse brain tissue was prepared at the University of Medicine and Dentistry of New Jersey and shipped to the University of Illinois at Urbana-Champaign for processing.

First, the brain tissue was prepared.

The brain tissue was prepared according toPontén et al. [ 20].