Sentence examples for brain tissue were lysed from inspiring English sources

N2a cells and brain tissue were lysed in RIPA buffer and denatured in Laemmli.

MIN6 cells, mouse islets, mouse exocrine tissue and mouse brain tissue were lysed in cell lysis buffer (Cell Signaling Technology, Danvers, MA, USA).

In brief, brain tissue was lysed in Mg2+ lysis buffer (Upstate) and mixed with GST-PBD or with GST-RBD bound to glutathione-agarose and incubated for 1 h at 4°C.

5 10 mg of brain tissue was lysed using cell lysis solution (Qiagen) and Proteinase K (MP Biomedicals LLC) was added and incubated at 55°C over night.

Frozen tumor and normal brain tissues were lysed in RIPA lysis buffer with protease inhibitors as described [34].

Rat brain tissues were lysed in homogenization buffer (20 mM Hepes, pH 7.6, 125 mM NaCl, 10% glycerol, 1 mM EDTA, 1 mM EGTA supplemented with 1% DTT).

For western blotting, cells as well as brain tissues were lysed in extraction buffer (Beyotime Inc., Shanghai, China) containing complete protease inhibitor cocktail (Roche, Indianapolis, IN, USA).

(B ) WT mice were dosed once intraperitoneally with AMD3100 at 10 mg/kg body weight and 24 hr later, the brain tissues were lysed in NP-40 buffer with phosphatase inhibitors and immunoprecipitated ZBTB16 antibody.

Pulmonary endothelial cells or lung tissue were lysed with RIPA buffer.

Islets, cells or tissue were lysed and analyzed by Western blot using specific antibodies.

Cells and cryosection of renal tissue were lysed with TRIZOL to isolate RNA.