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eluted with an identical retention time as synthetic IPAM (Figure 1c) by HPLC Initially, peaks corresponding to endogenous IPAM were inconsistent and could not be accurately quantified due to their very low basal concentrations which, in rat brain tissue were estimated to be below 100 pg indole/mg protein.
ATP, ADP and AMP levels in neurons and brain tissue were estimated using HPLC.
MPO levels in the peanut oil treated ischemic brain tissue were estimated to be 175 ± 5 mU/g/min, significantly higher as compared to the sham-operated group; 65 ± 5 mU/g/min.
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The diffusion coefficient for Aβ1 42 monomer in aqueous solution is available from the published literature and from this value the effective diffusion coefficient of Aβ1 42 monomer in brain tissue was estimated at 0.623×10−6 cm2/seesee Methods section).
The prior information for each class center of the three main brain tissues is estimated from 10 volumes.
Proinflammatory cytokine levels in brain tissue homogenates were estimated using CBA kits.
Hair and brain tissue were eliminated.
Brain tissue loss was estimated from the Nissl-stained brain sections in every fifth brain section (giving a distance of 150 μm between two sections).
Absolute brain metabolite tissue levels were estimated by using the brain water content as an internal reference.
Brain tissue volume was estimated with MIPAV software package, Medical Image Processing, Analysis, and Visualization (National Institute of Health, Bethesda, USA).
Examples of subcortical segmentation, after boundary correction, of a subject classified as MC and of a subject diagnosed with probable Alzheimer's disease, are presented in respectively Figs 1 and 2. Brain tissue volume was estimated with SIENAX (Smith et al., 2002), part of FSL (Smith et al., 2004).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com