H2 concentration in arterial blood, venous blood and brain tissue was detected after H2 inhalation separately.
An upregulation in gene expression of MCRs in the liver and brain tissue was detected in an acute-phase model induced by TO damage to skeletal muscle.
The water contents of the brain tissues were detected by measuring the ratios of the body weights and brain weights.
rats were sacrificed and the expression of Fas, FasL, Bcl-2, Bax as well as cleaved caspase-3, -8 and -9 proteins in brain tissues were detected by Western blot.
Carbonylated proteins of lung and brain tissues were detected and analyzed following derivatization of protein carbonyl groups with 2, 4-dinitrophenylhydrazine, using the OxyBlot kit (Millipore, Billerica, MA, USA).
IL-1β, TNFα protein and IgG in the brain tissues were detected by ELISA, following the manufacturer's instructions(Mouse IL-1β ELISA Kit/ Mouse TNFα ELISA Kit/ Mouse IgG ELISA Kit)(R&D Systems Inc., Minneapolis, MN USA).
3-OMD, a major metabolite of L-DOPA formed in peripheral and brain tissues, was detected at high levels in the plasma as well as cerebral spinal fluid of PD patients treated with L-DOPA/carbidopa [43], [44], and the plasma levels of 3-OMD in patients with dyskinesia were significantly higher than those from patients without dyskinesia [45].
Based on the available literature that the expression of miR-494-3p miR-494-3p miR-494-3pTEN mightand could suppress the expression of PTEN mRNA, the expression of the gene in 35 human glioma tissues and 8 normal beain targets was detheted.
Autoantibodies against neural cells and brain tissue were also detected in the plasma of ASD children [ 85– 85].
