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The entire slice of brain tissue was soaked in a form of inert glutamate, and the laser activated the chemical in precisely the area the scientists were focusing on.
Using 3 different doses of quinacrine (4 mg/kg/d, 80 mg/kg/d, 160 mg/kg/d), mouse brain tissue was soaked in 100% methanol for 7 days.
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After displacement, the tissue was soaked in Epon 812 epoxy resin and embedded.
Afterwards tissue was soaked in methylmethacrylat monomer, nonylphenyl-polyethyleneglycol acetate and azoisobutyronitrile (all Sigma-Aldrich, St . Louis USA).
For positive control, freshly dissected tissue was soaked in 2N HCl for 30 min to induce apoptosis.
The slides of hepatic tissue were soaked in 3%H2O2-methanolH2O2-methanolr 20 min in order to block endogenous peroxydase.
The tissues were soaked for 30 minutes in a solution of iron nitrate (FeIII NO3 3.9H2O, 7.65 g in 1 L water).
Tissues were soaked in low temperature paraffin (melting point of 52 54°C) overnight and embedded.
The frozen mouse tumor tissues were soaked overnight in RNAlater-ICE buffer (Ambion) before RNA extraction.
Decalcified tissues were soaked in 20 % sucrose/PBS solution overnight and embed in optimal cutting temperature compound (OCT) (Tissue-Tek, Torrance, CA, USA) and stored at –80 °C.
Brain tissue was centrifuged after flushing blood out of the brain.
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