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Brain tissue was sectioned, hematoxylin and eosin stained (H&E) and analyzed in a blinded procedure by a neuropathologist (TO) for presence and grade of tumors.
PN 21 and adult brain tissue was sectioned at 45 μm in series of four.
At the end of the MRI, animals were sacrificed and the brain tissue was sectioned following the procedure described above for tissue loss determination.
After fixation, the brain tissue was sectioned into 50 µm tissue slices using a Leica VT1000S vibratome (Leica, Wetzlar, Germany) and immunohistochemistry was performed as described by Gogolla et al. [ 22] with minor modifications.
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After 3 weeks of tumor growth, brain tissues were sectioned and H&E staining was performed.
Human and mouse brain tissues were sectioned on a cryostat (Jung CM3000; Leica, Richmond Hill, ON, Canada) at 10 μm thickness, and then mounted on Superfrost Plus microscope slides (Fisher Scientific, Nepean, ON, Canada).
> -wrap-foot> aRelated by common ancestry (Fig. 2) bReference for fresh brain weights [ 12] cReference for fixed brain weights [ 15] Formalin-fixed, paraffin-embedded tissue was sectioned at 6 μm and mounted on organosilane-coated slides (SIGMA, St Louis, MO, USA).
Frozen tissue was sectioned at 7 microns.
The wax-embedded brain tissue was then sectioned coronally into 3 μm sections using the rotary microtome, Microm HM315.
Brain tissue was fixed, sectioned, and mounted for brain region specific analysis of EB.
Brain tissue was collected and sectioned in the horizontal plane perpendicular to the axis of the microelectrodes.
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