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Brain tissue was processed as previously described [47] (see Materials and Methods S1).
Human brain tissue was processed as described [13].
Mouse brain tissue was processed as described [34] and staining procedure for human tissue was applied.
Mice were then sacrificed and their brain tissue was processed for Nissl staining.
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Cells and mouse brain tissues were processed as previously described [1], [20].
Brain tissues were processed and immunostained as described.
Rodent brain tissues were processed for hematoxylin eosin (H&E) and Congo red staining.
At 3 months post-transplantation, the mice were killed and the brain tissues were processed for human-specific cell identification.
Adjacent coronal cryostat sections of rat and human brain tissues were processed at −20°C and were thaw-mounted on superfrost plus microscopic slides, dried and stored as described earlier [ 54, 64].
Tissue was processed within 2 hours.
All brain tissue was routinely fixed, processed into paraffin wax, sectioned and stained with haematoxylin and eosin as described in detail elsewhere [ 16].
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