Sentence examples for brain tissue was performed from inspiring English sources
Extraction of Aβ from brain tissue was performed as described [30].
Immunoprecipitation-Western analyses of MDM2 in whole brain tissue was performed exactly as described in [38].
Maceration of the brain tissue was performed using a 10% solution of potassium hydroxide until only the resin casts of blood vessels remained.
Brain homogenates were separated into cytosolic and membrane fractions by centrifugation at 100,000×g for 1 hr at 4C. Isolation of lysosomal fractions from brain tissue was performed by differential centrifugation in a sucrose-based buffer essentially as previously described [69].
Purification of lysoPLD from rat brain tissue was performed exactly as described previously [ 21].
Immunofluorescent staining of brain tissue was performed using cryopreserved 40 μm coronal sections.
The biochemical analyses of TDP-43 in the sarkosyl-insoluble fraction from human brain or iGFP-WT and iGFP-NLSm transduced cells and in the RIPA-insoluble fraction from mouse brain tissue were performed on 12% Bis Tris gels (NuPAGE Novex, Thermo Scientific Inc., Rockford, IL) using MOPS-SDS as a running buffer.
To validate this presumption, triple immunofluorescent labelings of isoQC, CCL2 and GFAP on well-preserved human brain tissue were performed.
3D Reconstruction and quantification of the bdLV-transduced brain tissue were performed using a Leica DM 4000B microscope attached to a Neurolucida computer-assisted tracing system (Microbrightfiled, Inc., USA).
Then, comparisons between all groups of mice, analyzed for the presence of Cpn DNA in brain tissue, were performed with three-way ANOVA with Treatment (Cpn infection or mock infection), Time-point of analysis (1 week, 1 month or 3 months p.i). and Brain region (olfactory bulbs, cerebellum, hippocampus, subventricular zone or neocortex) as fixed factors.
