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DNA from blood samples was extracted with the QIAamp® DNA Mini Kit (Qiagen Inc., Valencia, CA, USA) and DNA from scrapie infected brain tissue was extracted using Chelex 100 chelating resin (Bio-Rad Laboratories InCA, Valencia, CA, USA) and phenol-chloroform extraction method [ 36].
Immediately following death, brain tissue was extracted.
Total RNA from brain tissue was extracted using Trizol reagent following the manufacturer's instructions.
Firstly, brain tissue was extracted prior to tissue-type segmentation using BET [ 29].
In short, whole brain tissue was extracted and minced before being crosslinked in 1% paraformaldehyde (PFA, Sigma-Aldrich) for 15 min at room temperature.
Genomic DNA from tumor and normal brain tissue was extracted by using the DNeasy Blood and Tissue Kit (QIAGEN) according to the manufacturer's instructions.
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Total RNA from patient neurons and brain tissues was extracted with the RNeasy kit (Qiagen) according to the manufacturer's instructions.
Brain tissues were extracted and dissected from Tsc2‐Olig2 CKO and littermate controls as described previously.
Proteins from these six brain tissues were extracted and solubilized using 0.2% RapiGest (Waters Corporation, Milford, USA) in 50 mM ammonium bicarbonate.
Mouse brain tissues were extracted in RIPA buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 2 mM EDTA, NP40 1%, SDS 0.1%) plus protease inhibitors (complete EDTA-free protease inhibitors, Roche Diagnostic, Milan, Italy) using a teflon dounce homogenizer (Wheaton, Millville, NJ, USA).
Blood clots and damaged tissue were extracted from inside the brain.
More suggestions(16)
brain material was extracted
brain tissue was considered
brain tissue was counted
brain tissue was centrifuged
brain mRNA was extracted
brain tissue was provided
brain area was extracted
brain tissue was sent
brain tissue was prepared
brain region was extracted
brain tissue was littered
brain tissue was stored
brain volume was extracted
brain tissue was processed
brain tissue was classified
brain tissue was obtained
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