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Immunohistochemistry was performed on frozen sections of brain tissue to detect kif21b protein.
Moreover, the authors suggest that blood might hold promise as a surrogate for brain tissue to detect DNA methylation in patients and as a source for biomarker discovery.
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Here we use high-throughput RNA sequencing of a single Drosophila melanogaster tissue to detect brain-specific differences in gene expression between the sexes and between two populations, one from the ancestral species range in sub-Saharan Africa and one from the recently colonized species range in Europe.
While natural oAβ can be readily identified in aqueous extracts of AD brain tissue, attempts to detect soluble oAβ in cerebrospinal fluid (CSF) have led to mixed results, with a few laboratories reporting their ready detection but others concluding that their levels are very low or virtually undetectable (discussed in [ 3]).
IL-1β, IL-6, and TNF-α proteins expressed in the brain tissue were detected by immunostaining, according to the standard streptavidin-biotin-peroxidase technique.
An upregulation in gene expression of MCRs in the liver and brain tissue was detected in an acute-phase model induced by TO damage to skeletal muscle.
H2 concentration in arterial blood, venous blood and brain tissue was detected after H2 inhalation separately.
To explore the underlying mechanisms, the expression level of apoptosis, inflammation and autophagy-related markers in brain tissue were detected with kits or by western blot.
In that study, however, pandemic (H1N1) 2009 virus was not isolated from the patient's cerebrospinal fluid (CSF) or brain tissue or detected by reverse transcription PCR (RT-PCR).
Alternatively, soluble mutant huntingtin HSJ1a complexes may be rapidly degraded in mouse brain tissue making them difficult to detect by co-immunoprecipitation.
Autoantibodies against neural cells and brain tissue were also detected in the plasma of ASD children [ 85– 85].
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