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Specifically, data obtained in human brain tissue sections from Alzheimer's disease patients showed that the expression of cannabinoid receptors of the CB2 type is induced in activated microglial cells while fatty acid amide hydrolase (FAAH) expression is increased in reactive astrocytes.
Therefore, we have used fixed brain tissue sections from wt and Sim1-/ at P0 and performed in situ hybridization for Pet1 (Figs. 7B-7E), RGS4 (Figs. 7F-7I) and Brn3.2 (Figs. 7J-7M).
To standardize sampling from animal to animal, coronal brain tissue sections from all animals were prepared from the same location in the basal ganglia, 5 mm posterior to the head of the caudate nucleus.
Immunohistochemistry of brain tissue sections from Mecp2-/y mice may be employed to test the hypothesis of a more pronounced growth impairment in astrocytes vs. neurons, although correct quantitation of astrocytes may be rather difficult for reasons outlined elsewhere [38].
Figure 7B shows the IHC image analysis for IBA-1 of brain tissue sections from the frontal cortex of 1) the uninfected, CD8-depleted control cohort, 2) the SIV-infected, untreated, CD8-depleted cohort and 3) the four SIV-infected, MN-treated, persistently CD8-depleted animals.
Brain tissue sections from each group were H&E stained and examined under the light microscope on day 5.
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HS-68 was reported to display a broad variation in emission spectra when bound to Aβ deposits, as well as to tau aggregates, in human AD brain-tissue sections,[ 29] therefore the probe was applied to brain-tissue sections from transgenic mice with Aβ (APPPS1 mice) or tau (P301S tau mice) pathology.
Paraffin-embedded brain tissue sections were obtained from different parts (temporal, frontal and occipital lobes, hippocampus, amygdala, and pre- and postcentral gyruses) of the brains of seven patients with AD, or from the corresponding parts of the normal brain (BioChain Institute, Newark, CA, USA).
An alternative approach to profiling microglia that is being explored involves laser capture microdissection (LCM) of microglia from brain tissue sections followed by gene expression profiling.
After 1 h of FNS in rats subjected to 2 h of MCAO and various times (3 h, 6 h, 12 h, 24 h or 72 h) of reperfusion, brain tissue sections (approximately 2 mm thick) from the point behind the optic chiasm in the ipsilateral cortex were quickly dissected and harvested on ice.
There was no evidence for expression of pirica in the brain from ISH on brain tissue sections.
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