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Viral genome in brain tissue samples was examined by heminested pan-flaviviral reverse transcription PCR (7 ).
One recognized limitation of this approach is that the same reference set of 15 non-malignant brain tissue samples was used in all of the SAM analyses.
In agreement with this, sphingosine kinase activity in our human brain tissue samples was much higher in the presence of high salt, which inhibits SphK1, than in the presence of TritonX-100, which inhibits SphK2.
The Aβ1 40 level in the brain tissue samples was interpolated from a standard curve generated by serial dilution of recombinant human Aβ1 40 (Sigma Aldrich) in the range 16,000 to 1.024 nM.
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At present, brain tissue samples are owned by the researchers or organizations collecting the tissue or doing the science.
The frozen brain tissue samples were weighed and homogenized in acidified butanol.
Brain tissue samples were formalin-fixed and paraffin-embedded, followed by cutting and haematoxylin eosin (HE) staining at the local institutes.
From cross-data comparison, it is clear that aging gene network of aged brain tissue samples is relatively similar to that of Dlx2+/Dcx+ cells, and to a lesser extent, cultured NSC/NPCs (Fig. S4), suggesting that in brain tissues surrounding EZ-SEZ/SVZ, Dlx2+/Dcx+ NB might represent one of the major cell populations.
The highest levels of S1PR2 in human brain tissue samples were in two female MS patients who had "relapsing and remitting MS", meaning their symptoms flared irregularly.
Harvested brain tissue samples were digested into single cell suspension using papain as described in the methods.
Briefly, brain tissue samples were homogenized in 2∶1 chloroform:methanol containing 0.5% butylated hydroxytolulene and lipids were extracted by the method of Folch [58].
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