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All FAT-positive bat brain tissue samples collected from 1997 to 2003 tested positive by PCR.
Gene expression was then measured in brain tissue samples collected from these cows, 14 of which were sacrificed at the start of estrus and 14 around mid cycle.
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A limited amount of vitreous humor was collected; brain and other organ tissue samples collected were fixed in formalin.
The tissue samples collected were brain, lung, heart, liver, kidney, adrenal gland, jejunum, testis, ovary, and uterus, which were used to analyze the tissue expression pattern of CYP2C93 mRNA.
RNA sequencing (RNA-seq) data was generated from 524 tissue samples collected from 42 post-mortem brains collected from neurologically unremarkable individuals spanning early pre-natal development (8 post-conception weeks, PCW) to late adulthood (40 years of age).
The tissue samples collected from the heart, lung, muscles, brain, kidney, liver, and intestine were analyzed and examined histologically.
The tissue samples collected from adjacent region of tissue blocks were prepared as both FF and FFPE samples following the usual protocol in National Health Service diagnostic laboratories.
Tissue samples collected at each time point included plasma, red blood cells (RBCs), liver, lungs, kidneys, spleen, muscle, thymus, fat, brain, skin (ear) and bones.
All brain tissue samples were collected post-mortem.
As part of this surveillance, from 1984 to 2003, brain tissue samples were collected from all submitted bats.
Blood, lung, and brain tissue samples were collected from the rodents.
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