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Brain tissue from each animal will be sent for testing to the National Veterinary Services Laboratory in Ames, Iowa.
In brief, brain tissue from each animal was homogenized and subjected to proteinase K (PK) treatment for 1 h at 37°C.
A 10% wt/vol crude homogenate of brain tissue from each animal was tested for infectious arenavirus by cultivation in a monolayer of Vero E6 cells (4).
Around 100 mg of frontal lobe brain tissue from each individual were homogenized by crushing in liquid nitrogen with the aid of a mortar and pestle.
250 mg of fresh-frozen brain tissue from each region was available for protein extraction from ten controls, 9 PD and 6 pure DLB cases.
A steel mortar and pestle chilled in dry ice were used to pulverize the frozen brain tissue from each subject separately into a dry homogenate in the presence of liquid nitrogen.
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A quantitative polymerase chain reaction (QPCR) was used to identify if HIV DNA was present in significant amounts in the brain tissues from each patient.
Brain tissue from ten bees was used from each of the two cohorts (trained and control groups) for RNA analysis.
Researchers also are examining frozen brain tissue from birds that died as long ago as last fall.
It appears that the new brain tissue from the fetal cells overgrew and began pouring out too much dopamine.
During that time, Dr. Gambetti and his colleagues have examined brain tissue from nearly 500 Americans who died from unusual neurological conditions.
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