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In addition, the microglia priming network was also analyzed using (unsorted) brain tissue expression data.
WGCNA is often used to generate microglia specific profiles in brain tissue expression data [ 1, 19, 21].
In brain tissue expression data, a consensus green module was found, that is significantly up-regulated with aging and neurodegeneration.
These data suggest that signatures of primed, but not acute activated, microglia are preserved in brain tissue expression data from models of Alzheimer's disease, prion infection and, aging.
As a consequence, typical hub genes of microglia modules identified using brain tissue expression data are not necessarily hub genes in pure microglia expression data.
The overlap between the up-regulated blue modules in pure microglia and significantly up-regulated genes in brain tissue expression data was determined as a measure of preservation.
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In normal brain tissue, expression of hNinein isoforms 1, 2, 5, and 6, γ-tubulin, and centrin 3 was rare whereas Aurora A, Aurora B, and centrin 2 mRNA expression was absent (data not shown).
Brain gene expression data also provides ample comparisons with previously published findings, mostly focusing on brain tissue gene expression in primitively and advanced eusocial species [ 9, 18].
Because human brain expression data is derived from post-mortem brain tissue, special attention must be paid to RNA quality, post-mortem interval, and pH.
qRT-PCR also demonstrated that expression of type-2 PTH receptor was undetectable in all tested myeloma cell samples, although it was highly expressed in human brain tissue (data not shown).
The brain expression data also revealed expression of transposable elements in the cichlid brain in E. cyanostictus, suggesting them to be active in this tissue.
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