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Blocks of brain tissue containing the noradrenergic LC nucleus and two nuclei of dopaminergic neurons, the SN and VTA, were sectioned and sampled in a systematic-random manner and immunostained for tyrosine hydroxylase (TH), a specific marker for catecholaminergic neurons.
Slices of brain tissue containing the BNST were placed in a superfusion chamber and visualized with a Leica stereomicroscope under low magnification.
Brain tissue containing the EWN (Talairach space 28 29 mm) according to [ 31], the LC (Obex 20 32) according to [ 42] and the NbM (Ch4; Talairach space −2 to −0.6 mm) according to [ 2, 31] from the same cases was used for immunohistochemistry to detect the presence of QC, pE-Aβ and neuronal markers.
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Serial coronal sections (30 μm) were cut from the rostal to caudal edge of the brain tissues containing the tumours by using a cryo-microtome.
Freshly dissected brain tissues containing the amygdala were processed for staining individual neurons using the rapid Golgi method (Vyas et al, 2002; Mitra et al, 2005; Mitra and Sapolsky, 2008; Mitra et al., 2009c).
Intriguingly, the cDNA libraries from developmental (brain) tissues contain the highest amount of ncRNA candidates, about two percent.
We evaluated the accuracy of NeuronStitcher using carefully generated "ground truth" reconstructions from a piece of mouse brain tissue containing a labeled pyramidal neuron within the hippocampal CA1 region (Supplement, Section 4.1).
The new paper, which describes research done at the Krasnow Institute for Advanced Study at George Mason University in Fairfax, Va., and financed by the National Institutes of Health and the Whitaker Foundation, took as its starting point earlier work showing that electric fields could affect the firing of individual neurons or slices of brain tissue containing many neurons.
Equal amounts of brain tissue containing bradyzoites in tissue cysts from three chronically infected sheep was homogenized and fed to cats (55 g per cat).
We designed this simulation tool to be easily adaptable to create models of any layered brain tissue containing populations of spiking neurons (Fig. 3).
However, limited resources appear to be available for classical developmental neuropathology, with inadequate networks to facilitate access to post-mortem brain tissue containing malformations of cortical development.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com