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Protein and mRNA levels of complex I subunits, 51-, 24- and 75-kDa, were analyzed in two brain specimens obtained from the striatum and the lateral cerebellar hemisphere (Fig 1) of patients with schizophrenia, bipolar disorder, major depression and normal subjects.
Negative controls included omission of primary antibodies and sections of age-matched normal brain specimens obtained from the Department of Pathology, BC Children's Hospital (Vancouver, BC, Canada).
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To analyze the expression of MMP14 in glioma patient's tissues, we obtained a tissue microarray (TMA) provided by US Biomax (Rockville, MD) and supplemented these data by the staining of brain tumor specimens obtained from patients undergoing brain tumor resection at the University of Chicago Medical Center.
Fresh human astrocytoma (Grades I IV) and non-malignant brain tissue specimens obtained from epilepsy reduction surgery were collected under a North Shore University Hospital's Institutional Review Board-approved protocol.
Tissue specimens obtained from the brain and spinal cord of affected cattle were rinsed, minced and homogenized in serum-free Eagle's minimum essential medium (MEM) (Nissui Pharmaceutical Co., Tokyo, Japan).
He based his account on specimens obtained from Jamaica, though no type specimens were designated.
Among these, 44 specimens were CaP-benign paired specimens obtained from the same patient.
Brain specimens were obtained from the Queen Square Brain Bank, London and the Institute of Psychiatry, London.
Postmortem human brain specimens were obtained from tissue collections at the Department of Neurobiology at Yale University School of Medicine and the Clinical Brain Disorders Branch of the National Institute of Mental Health.
Normal breast tissues were obtained from the periphery of specimens surgically resected for treatment of cancer or from an autopsy specimen obtained from a patient who had died of injuries sustained in an accident (not breast cancer); the latter was obtained from the Brain and Tissue Bank for Developmental Disorders at the University of Maryland.
Brain tissue specimens obtained by autopsy from these patients may be submitted to the NPDPSC for further analysis and confirmation of the CJD diagnosis.
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