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Brain specimens containing the right LGN were cryoprotected, frozen and serially sectioned (50 μm thick) with a sliding microtome in the coronal plane perpendicular to the optic tract.
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Biodistribution studies in tumor-bearing mice showed that brain specimens contained more label at 10 min than the tumors but less at 30 or 60 min. Brain uptake relative to the tumors, the brain/tumor ratio, ranged between 0.5 and 3.3.
While some of the specimens contained the familiar strain of flu known as A/Victoria, others contained a strain of influenza A that could not be identified.
Thus, brain-specific microRNAs, such as hsa-miR-124, can be found variously in tissue specimens containing brain metastases, and their expression levels measured in bulk tissue samples do not allow discrimination between brain primary tumors and metastases located in the brain.
(A ) A low-resolution (20×) confocal stack covering the entire brain and optic lobes (magenta diagram) and a single high-resolution (63×) confocal stack of the portion of the brain containing the MB (blue square) are collected for each specimen.
All biopsy specimens contained residues of the substitute material.
We first diagnosed a patient presenting dementia and obesity whose brain biopsy and cerebrospinal fluid specimens contained T. whipplei DNA and who responded dramatically to antibiotic treatment.
None of the specimens contained any mutations at positions A2067 or C2617.
190/200 (95%) of the specimens contained >10% tumour tissue.
The primary antibody was omitted in the negative control and sections from Microcebus murinus brain specimens known to contain ß-amyloid plaques were used as positive controls.
Anne D. Williams, the curator of the jigsaw puzzle portion, stressed how much educational lore the early specimens contained.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com