Sentence examples for brain slides were fixed from inspiring English sources

Fluorescence double labeling: Eyes and brain slides were fixed in 100% ethanol for 2 h.

For immunohistochemistry, coronal brain sections, cryosectioned at 25 μm and lifted on Superfrost+ microscope slides, were fixed in 4% PFA for 15 min and rinsed twice with 1× PBS for 5 min each.

Embryos attached to slides were fixed and stained as described above.

L1s attached to slides were fixed and stained as described above.

Slides were fixed in cold MeOH (4 °C) for 20 min prior to staining.

Briefly, frozen slides were fixed in Bouin's solution.

Paraffin slides were deparaffinized as previously described while the frozen slides were fixed for 5 minutes in 80% methanol.

For immunofluorescence staining, cryosections of frozen livers were performed and slides were fixed with acetone.

Before incubation, the slides were fixed using 0.1% PFA and washed in Tris buffer.

Slides were fixed with formalin 4% in PBS for 15 minutes and permeabilized.

Air-dried slides were fixed in 2% paraformaldehyde (Sigma) for 20 minutes.