Sentence examples for brain slices were washed from inspiring English sources

The incubated brain slices were washed in acidified KRB (pH = 5.0±0.2), rinsed 3 times with ice-cold KRB, and imaged.

Free-floating brain slices were washed in Tris-buffered saline (TBS) and pre-incubated in 3% goat normal serum (NGS) in TBS for 30 min. Cells were also fixed with 4% formaldehyde and rinsed in TBS.

At the end of the experiment, all the brain slices were washed in acidified KRB (pH = 5.0±0.2), rinsed 3 times with ice-cold KRB, and assayed for the radioactivity in a dual channel gamma counter (Perkin-Elmer, Waltham, MA).

Finally, the brain slices were washed in PBS and mounted in Mowiol mounting solution (Mowiol 4 88).

For labelling of DCX, brain slices were washed in PBS before incubation with 0.1% Triton X-100/PBS for 15 min on ice.

Brain slices were washed in 0.2 M sodium acetate and developed in DAB + nickel ammonium sulfate (17mg of DAB, 1.25 g Nickel ammonium sulfate in 50 ml of 0.2 M sodium acetate and 0.3% H2O2).

Slices were washed and embedded in Mowiol (Calbiochem).

Slices were washed in sodium acetate and TBS and mounted.

Hydrochloric acid alcohol was added for 1 minute and then slices were washed for 25 minutes.

Subsequently, the slices were washed twice with Krebs solution, and the protocol was started.

Slices were washed with water and processed for embedding in paraffin.