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After a 5-h recovery at 37 °C, brain slices were used for quantification of cell injury by spectrophotometric measurement of formazan produced from 2,3,5-triphenyltetrazolium chloride.
7,000 of these thin human brain slices were used to create the BigBrain.
In some experiments, human brain slices were used under the approval of the ethical committee at Tokyo Women's Medical University (approval number #1147).
(1519) Brain slices were used to analyze metabolism(1885) and the mechanisms of learning and addiction.
The frozen brain slices were used for verification of the recording and optical stimulating sites by observing the different optrode traces inside the slices under a microscope.
At 5 weeks post injection (5wpi), animals were killed and dissected brain slices were used for morphological and proliferative analysis by confocal microscopy.
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A Siemens/CTI 931/08-12 scanner with the capability of 15 horizontal brain slices was used.
Can other intracellular or extracellular parameters measured in acute brain slices be used to sort the population into groups?
To achieve this, one rat or three mouse brain slices are used after the 5 h incubation for each experimental setup.
Extracellular recording of DAergic VTA neurons in brain slices was used to test the effects of ethanol (EtOH) (10 to 80 mM) on DIR.
A minimum of three replicate slices were used for each brain per experiment.
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